Population Genetics of Tidestom's Lupine (Lupinus tidestromii var. tidestromii)


SCH Number
Public Agency
California Department of Fish and Wildlife, Habitat Conservation Planning (CDFW)
Document Title
Population Genetics of Tidestom's Lupine (Lupinus tidestromii var. tidestromii)
Document Type
NOE - Notice of Exemption
Document Description
The California Department of Fish and Wildlife is issuing a permit to Aspen Workman (Permit No. 2081(a)-23-001-RP) pursuant to Fish and Game Code section 2081(a) for a project to improve the population genetic understanding of Tidestrom’s lupine. Results are expected to inform future habitat management decisions to conserve the genetic integrity of Tidestrom’s lupine. Plant tissue collection activities will take place between March and April of 2023. Three to five leaves will be collected from fifteen to twenty individuals at each location. At five sites where Tidestrom’s lupine co-occurs with Chamisso bush lupine (Lupinus chamissonis), putative hybrids will also be sampled totaling up to twenty leaf tissue collections per site. Leaves will be pinched off the stem at the base of the petiole, placed in envelopes and stored on silica gel until DNA extraction activities can be performed at Helmoltz Center for Environmental Research GmbH- UFZ in Germany. Each individual plant will be photographed, and GPS coordinates will be recorded. Data on reproductive status and morphology of the plant may be recorded to inform hybridization analyses. Plant tissue DNA will be extracted using a Qiagen DNEasy plant kit and samples will be processed using sequence-capture methodology. Following analysis, sequence data will be uploaded to public repositories.

Contact Information

Joanne Heraty
Agency Name
California Department of Fish and Wildlife, Habitat Conservation Planning Branch
Job Title
Senior Environmental Scientist- Specialist
Contact Types
Lead/Public Agency


Marin, Monterey, Sonoma
Other Information
Plant material collection for the Population Genetics of Tidestrom’s Lupine (Lupinus tidestromii var. tidestromii) project (Project) will take place at Asilomar State Beach and Conference Grounds State Park (36.6227, -121.9393), North Dunes at Carmel Beach (36.5559, -121.9292), Pacific Grove Golf Links (36.6355, -121.9356), The Links at Spanish Bay (36.6116, -121.9459), Spyglass Hill Golf Course (36.5811, -121.9654), and The Sanctuary Beach Resort (36.7026, -121.8066) in Monterey County, CA. Collections will also take place at Point Reyes National Seashore (38.0785, -122.9730) in Marin County, CA and Sonoma Coast State Park (38.4473, -123.1260) in Sonoma County, CA. DNA extraction and sequencing will be conducted at Helmoltz Center for Environmental Research GmbH- UFZ in Halle, Germany.

Notice of Exemption

Exempt Status
Categorical Exemption
Type, Section or Code
California Code of Regulations, Title 14, Section 15306, Class 6
Reasons for Exemption
The project consists of basic data collection and research that will facilitate the genetic understanding of Tidestrom's lupine. The permit issued by the California Department of Fish and Wildlife for the Project includes measures to ensure that existing populations of Tidestrom's lupine are not detrimentally impacted by research activities.
County Clerks
Marin, Monterey, Sonoma


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