The California Department of Fish and Wildlife is issuing a permit to the United States Fish and Wildlife Service (USFWS) (Permit No. 2081(a)-22-010-RP) pursuant to Fish and Game Code section 2081(a) for a project to study conditions and techniques for successful in situ germination and population reestablishment of Scotts Valley polygonum in Santa Cruz County. The Project will directly address the 2020 USFWS recommended recovery action to outplant Scotts Valley polygonum to historical locations using bulked seed. The initial pilot study will take place at Polo Ranch. Four quadrats with different treatments will be placed at four historic locations, for a total of 16 quadrats. Approximately 8,000 seeds produced from greenhouse-reared plants under authority of CESA permit 2081(a)-18-004-RP will be used as source material. In the fall/winter of 2022/2023 approximately 500 seeds will be broadcast into each quadrat. Photos will be taken before and after planting. Planted areas will be covered with mesh to reduce herbivory and grazing exclosures will be installed. Maintenance of the quadrats will include hand weeding, thatch removal and maintenance of herbivory mesh. Monitoring will be conducted monthly beginning in February 2023 to assess germination of seedlings, flowering, fruiting, mortality, evidence of herbivory, or other conditions affecting outplanting success. Water treatments will be applied at each monitoring event unless natural precipitation is considered adequate. Results from the initial pilot study at Polo Ranch will inform future reintroduction efforts that are expected be continued by the Permittee, assistant investigators, and partners of the Scotts Valley Polygonum Recovery Implementation Team with the goal of establishing additional self-sustaining populations.