Joshua Tree Genome Project

Summary

SCH Number
2022050469
Public Agency
California Department of Fish and Wildlife, Habitat Conservation Planning (CDFW)
Document Title
Joshua Tree Genome Project
Document Type
NOE - Notice of Exemption
Received
Posted
5/20/2022
Document Description
The California Department of Fish and Wildlife is issuing a permit to Jeremy Yoder [Permit No. 2081(a)-22-004-RP] pursuant to Fish and Game Code section 2081(a) for a project to collect Joshua tree tissue and flower samples to provide genomic data for an annotated reference genome. Genetic resources will provide an understanding of Joshua tree’s coevolution with their specialized pollinators and adaptation mechanisms to extreme desert climates. A small amount of fresh, green leaf tissue (approximately 100 grams) and ten fresh flowers will be collected from 100 individual plants at each of the thirteen plant material collection locations. Plant tissue DNA will be extracted using a Qiagen DNEasy plant kit and samples will be processed using sequence-capture methodology targeting loci with known or suspected roles in climate adaptation and floral development. DNA will be prepared into libraries for multiplex sequencing and aligned to the draft Joshua tree reference genome. Data will be made publicly available on the National Center for Biotechnology Information (NCBI) Sequence Read Archive following scientific publication. Floral samples will be exported to Willamette University for dissection and measurement analysis.

Contact Information

Name
Joanne Heraty
Agency Name
California Department of Fish and Wildlife, Habitat Conservation Planning Branch
Job Title
Senior Environmental Scientist- Specialist
Contact Types
Lead/Public Agency

Location

Counties
Kern, Los Angeles, San Bernardino
Regions
Countywide

Notice of Exemption

Exempt Status
Categorical Exemption
Type, Section or Code
California Code of Regulations, Title 14, Section 15306 Class 6
Reasons for Exemption
The project consists of basic data collection and research that will facilitate the genetic understanding of Yucca brevifolia. The permit issued by the California Department of Fish and Wildlife forthe Project includes measures to ensure that existing populations of Yucca brevifolia are not detrimentally impacted by research activities.

Attachments

Notice of Exemption

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