Molecular Profile and Ecological Analysis of Arctostaphylos densiflora Project
- SCH Number
- Lead Agency
- Fish and Wildlife (Headquarters), Habitat Conservation Planning Branch - Sacramento (California Department of Fish and Wildlife, Habitat Conservation Planning )
- Document Title
- Molecular Profile and Ecological Analysis of Arctostaphylos densiflora Project
- Document Type
- NOE - Notice of Exemption
- Project Applicant
- Dr. Christopher Baysdorfer, Director, Green Biome Institute
- Present Land Use
- California Native Plant Society’s Vine Hill Preserve in western Sonoma County, California
- Document Description
- The California Department of Fish and Wildlife is issuing a permit to the Green Biome Institute, California State University East Bay (Permit No. 2081(a)-20-002-R pursuant to Fish and Game Code section 2081(a) for a project to sequence and annotate DNA of Arctostaphylos densiflora, a state-listed endangered plant, and conduct an ecological analysis of the species’ habitat. Project activities include genetic sequencing, soil seedbank analysis, germination tests, and identification of seed predators. Five to ten flowers or one gram of material and a single leaf will be collected from each plant in the population and genomic DNA will be sequenced and assembled in the Green Biome Institute research laboratory. For soil seedbank analysis, a total of eight soil samples will be collected from beneath the canopy of different Arctostaphylos densiflora plants. The soil samples will be analyzed to determine the parent material and nutrient content and will be used for germination testing. Following analysis, any residual soils will be bagged and kept in dry storage at the Green Biome Institute. Germination testing will also be conducted from seeds collected from individual plants. Five to ten ripe fruits, each containing one to several seeds, will be collected from each plant in the population, and the seeds will be cold stratified and tested for germination in the Green Biome Institute research laboratory. Only half of the collected seed will be used for germination testing, and the remaining seed will be stored in the Green Biome Institute’s sub-zero freezer for future testing if the initial germination study is unsuccessful. To identify seed predators of Arctostaphylos densiflora, two camera traps will be installed under the shrub canopy using small metal stakes, and data will be collected bi-weekly from August to October in 2020 or 2021.
- Contact Information
California Department of Fish and Wildlife
California Department of Fish and Wildlife P.O.Box 944209
Sacramento, CA 94244-2090
Phone : (916) 376-8676
- Northern California
- California State University East Bay Campus in Hayward
- Other Location Info
- California Native Plant Society’s Vine Hill Preserve in western Sonoma County, California. Studies will take place at the Green Biome Institute research laboratory on the California State University East Bay Campus in Hayward, California.
Notice of Exemption
- Exempt Status
- Categorical Exemption
- Type, Section or Code
- Section 15306, Class 6
- Reasons for Exemption
- The Project consists of basic data collection, research and resource evaluation activities and will not result in a serious disturbance to Arctostaphylos densiflora or any other environmental resource. The permit issued by the California Department of Fish and Wildlife for the Project includes measures to ensure that existing populations of Arctostaphylos densiflora are not impacted.
- Notice of Exemption
- C__Users_AKammet_Desktop_20-002-RP NOE PDF 221 K
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