The California Department of Fish and Wildlife is issuing a permit to the Green Biome
Institute, California State University East Bay (Permit No. 2081(a)-20-002-R pursuant to Fish and Game Code section 2081(a) for a project to sequence and annotate DNA of Arctostaphylos densiflora, a state-listed endangered plant, and conduct an ecological analysis of the species’ habitat. Project activities include genetic sequencing, soil seedbank analysis, germination tests, and identification of seed predators. Five to ten flowers or one gram of material and a single leaf will be collected from each plant in the population and genomic DNA will be sequenced and assembled in the Green Biome Institute research laboratory. For soil seedbank analysis, a total of eight soil samples will be collected from beneath the canopy of different Arctostaphylos densiflora plants. The soil samples will be analyzed to determine the parent material and nutrient content and will be used for germination testing. Following analysis, any residual soils will be bagged and kept in dry storage at the Green Biome Institute. Germination testing will also be conducted from seeds collected from individual plants. Five to ten ripe fruits, each containing one to several seeds, will be collected from each plant in the population, and the seeds will be cold
stratified and tested for germination in the Green Biome Institute research laboratory. Only half of the collected seed will be used for germination testing, and the remaining seed will be stored in the Green Biome Institute’s sub-zero freezer for future testing if the initial germination study is unsuccessful. To identify seed predators of Arctostaphylos densiflora, two camera traps will be installed under the shrub canopy using small metal stakes, and data will be collected bi-weekly from August to October in 2020 or 2021.