The California Department of Fish and Wildlife is issuing a permit to Benjamin Carter pursuant to Fish and Game Code section 2081 (a) for a project to study the population genetics, pollination and dispersal ecology, and germination success of Dudley's lousewort. For the population genetic analysis, a small amount of leaf
tissue will be collected from 125 individual plants from both the Little Sur River and Portola Redwoods State Park Populations, for a total of 250 samples. Pending landowner approval, the Permittee will collect an additional 10-20 samples from the population on the Hearst Ranch property. If landowner permission is not granted, the Permittee will collect tissue from herbarium sheets of the population housed at the Cal Poly San Luis Obispo herbarium. Additionally, three voucher specimens will be collected from each of the three populations. To test the effects of litter depth on seedling establishment, the Permittee will perform litter removal trials within Dudley's lousewort populations at the Portola Redwoods State Park Population. To determine the identity of seed dispersers and pollinators, a combination of time-standardized field observations and video recordings will be used during the flowering and fruiting season (mid-spring to mid-summer of 2019). Data will be collected from a single robust population in Portola Redwood State Park. To compliment video analysis, undergraduate student observers will perform time standardized observations of pollination and seed dispersal.