The California Department of Fish and Wildlife is issuing a permit to the Santa Barbara Botanic Garden (2081(a)-16-008-RP) pursuant to Fish and Game Code section n2081 (a) for a project to gather beach spectaclepod tissue samples during the course of range-wide survey efforts. Approximately 4-5cm of new, fresh, green leaf and/or bract tissue will be gathered from between 290 and 310 beach spectaclepod plants. Approximately 30 plants will be sampled from each of the nine sites and a total of 20-40 additional samples will be collected from populations that area large enough to tolerate additional sampling. Samples will be placed in envelopes that are placed in silica gel to rapidly desiccate the tissue samples and preserve DNA quality. DNA will be extracted from the samples at Santa Barbara Botanic Garden and DNA will exported to Floragenex laboratories in Oregon for restriction site associated DNA sequencing (RAD-seq) library preparation and sequencing.
The project will contribute to the recovery of the California threatened beach spectaclepod by investigating the population genetics of the species. This project will consist of basic data collection and research. The permit issued by the California Department of Fish and Wildlife for the project includes measures to ensure that existing populations of beach spectaclepod are not impacted.
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